Lab is specialized in chemical synthesis of complex glycans which are present on the surfaces of bacteria and viruses. These glycans can be conjugated with proteins for vaccine development.
For this approach, one needs to know the specific structures of the glycans. Small sugar based molecules can be synthesised, to be tested as virus entry inhibitors, if the details about the nature of host-virus interaction at molecular level are available
Look at standardizing and developing a chemical synthesis protocol for the anti-viral drug (Prof. Gudi ravigudi@iitb.ac.in, Prof. Mahajani sanjaym@che.iitb.ac.in, Prof. Wangikar wangikar@iitb.ac.in )
We can participate in the synthesis of anti-viral agents. Also we can provide structures of large number of compounds which can be subjected to virtual screening by computational methods to identify the lead compounds (I had a joint MSc project with Prof Prasenjit Bhowmik of BSBE) on similar approach.
We can also supply available compounds in good quantities if someone is interested in screening directly by experimental methods.
Design and large scale synthesis of anti viral agents
We are interested in synthesising anti-viral agents. New chemicals can be prepared by our methods and known anti-viral agents can be prepared in large scale. We can design and synthesize new anti-viral agents.
In collaboration with other research groups, we are interested in contributing generation and testing of various anti-viral agents which will be effective against COVID-19.
Our strength is to prepare new chemical entities in large numbers and quantities. We will be happy to write a proposal or can be part of a larger mission.
We can join with expertise in organic synthesis of compounds that can be tested. We have some compounds that worked well as anti-bacterials but not tested for antiviral. These can be tried for antiviral activity.
Have experience in all aspects of virology, from culture to cultivation to understanding their biology/evolution, detection of viruses, and therapy development.
In-situ Gel for Rapid Capture and Inactivation of 2019-nCoV
Nanoparticle based drug delivery, Structure analysis
Nanoparticle based drug delivery and formulation (Prof. Bellare; jb@iitb.ac.in)
Cryo-Electron microscopy to determine the structure of SARS-CoV-2 spike protein and its cellular receptor during infection. This could be helpful for design of new molecules or in decision on repurposing existing molecules.(Prof. Bellare; jb@iitb.ac.in)
Collaborators: Dr.Om Shrivastav, Jaslok Hospital Dr. Jayanthi S. Shastri, (T N Medical College & Nair Hospital) Specialist at Kasturba, Nair & Jaslok Hospitals.
Proteomic analysis
Proteomic analysis
Identification of global metabolite biomarkers in COVID-19 infected patients for targeted therapy
Plasma Proteomic Analysis of COVID-19 patients to identify the potential biomarkers and therapeutic targets
Microdevice based diagnostics, improved point f care diagnostics
Four microdevices have been developed which can potentially be integrated with an appropriate point-of-care or diagnostic device. These are:
Blood plasma separation microdevice
3d-Hydrodynamic Focusing
Constant temperature microdevice
Platelet-rich-plasma separation microdevice
There is great value in selectively isolating and enriching platelets in plasma from whole blood with application in biomedical research and transfusion purposes.
Has worked on technology to differentiate virus and bacteria. Rapid confirmative categorization of infections in clinical settings as bacterial or viral is frequently challenging due to the vague presentations of diseases and symptomatic similarities.
Many earlier studies have shown the bactericidal effect of silver nanoparticles which, in the current study has been enhanced by synergistic action of polycationic chitosan stabilizers.
An impedimetric electro-active polymer (polyaniline) modified paper substrate has been developed for generic differentiation of bacteria and virus based on their interaction with chitosan stabilized silver nanoparticles.
These characteristic electrical impedance signatures of bacteria and virus, established here, have promising prospects in inexpensive and scalable bedside diagnostic development.
Coronavirus has become major global threat as it is rapidly spreading across the world originating from China. There is no treatment exists which effectively control the viral spread.
Considering the severity of the novel coronavirus there is an urgent need to identify potent analogue / inhibitors which targets and controls the viral replication. Coronavirus proteases are considered as attractive targets for the design of antiviral drugs.
The high-resolution crystal structure of main protease with its inhibitor has been very recently determined using x-ray crystallography. Therefore, we used this structure (PDB ID: 6LU7.pdb) to investigate atomic level interactions between main protease of corona virus and existing protease inhibitors using molecular modeling approach.
Currently an ongoing projects to build blood cell counters, a diagnostic tool. These measure the complete blood count (CBC) - RBC, WBC and platelets. The number of WBC increases with a bacterial infection and hence is used to differentiate a bacterial infection from a viral infection. Further, the device can also detect virus infected cells, if one can synthesise fluorescent antibodies that will bind to virus infected cells. The latter is of course a challenge for COVID-19 and is a project in itself. Note that such blood cell counters are available in India but all of them are imported. The IMPRINT, GoI project was to develop indigenous technology.
Our recent efforts in the use of machine learning is applicable to drug discovery and process (such as catalytic reactions) related to efficient generation of such potentially active compounds.
We would like to use a combination of density functional theory computations to accelerate the process of identifying lead compounds.
Non-invasive and minimally-invasive devices for health monitoring, diagnosis an biotelemetry in portable, wearable and implantable applications with support features based on IoT; Team has demonstrated a full working prototype using in-house designed ASICs (application specific integrated circuits) with artifact-tolerant signal conditioning and record noise efficiency factor for multi-parameter multi-channel for both monitoring and pre-diagnosis purposes with MedRadio biotelemetry; working on the development of several size-reduced, indigenous and improved measurement devices used in the clinics by the doctors; Also works on machine learning based techniques for pre-diagnosis applications
Has worked on technology to differentiate virus and bacteria. Rapid confirmative categorization of infections in clinical settings as bacterial or viral is frequently challenging due to the vague presentations of diseases and symptomatic similarities.
Many earlier studies have shown the bactericidal effect of silver nanoparticles which, in the current study has been enhanced by synergistic action of polycationic chitosan stabilizers.
An impedimetric electro-active polymer (polyaniline) modified paper substrate has been developed for generic differentiation of bacteria and virus based on their interaction with chitosan stabilized silver nanoparticles.
These characteristic electrical impedance signatures of bacteria and virus, established here, have promising prospects in inexpensive and scalable bedside diagnostic development.
Sanitisation solution for hands for Institute personnel
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